Volume 2, Issue 6, November 2014, Page: 60-67
Seasonal Changes in the Dynamic State of Water for Excised Cherry Branches (Prunus lannesiana) Observed Using Dedicated Micro-Magnetic Resonance Imaging
Hiromi Kano, Oak-Hill Georgic Patch-Work Laboratory, Chiba, Japan
Mika Koizumi, Research Institute for Science and Engineering, Waseda University, Tokyo, Japan
Received: Nov. 22, 2014;       Accepted: Dec. 7, 2014;       Published: Dec. 17, 2014
DOI: 10.11648/j.plant.20140206.11      View  2664      Downloads  122
Seasonal changes in the dynamic state of water for cherry (Prunus lannesiana), a deciduous broad-leaved tree, were studied by combined k-space and q-space imaging using dedicated magnetic resonance imaging (MRI) with a 1.0-T permanent magnet. Water amounts, diffusion coefficients and transpiration were examined for excised branches cut from a tree with and without weak light (100–140 μmol m-2 s-1) throughout the year. The water amount in the cambium was large in spring and summer, decreased in autumn, and decreased further in winter. There were three components in the diffusion coefficient of the branch. The second component of the diffusion coefficient ascribed to the cambium did not fluctuate notably throughout the year, despite marked alternation in water amounts. However, diffusion coefficients in the secondary xylem, the primary component, were elevated in summer and decreased in winter. Upward water flow was restricted in the secondary xylem, and the positions where large flow was detected coincided with places exhibiting high diffusion coefficients and the arrangements of vessels. Total transpiration exhibited a tendency similar to that of the diffusion coefficients; however, total transpiration declined to zero when the plant had no leaf, whereas the diffusion coefficient decreased to 60 % of the maximum but did not decrease further. Light-enhanced transpiration related to potential photosynthetic activity increased in spring as the leaves sprouted and grew, considerably decreased in summer, decreased to one third of the maximum in autumn, and was not detected in winter. Measurement of the dynamic state of water for the excised branches will provide useful information for better understanding of the phenological changes of tree physiology.
Cherry Tree (Prunus lannesiana), Dedicated MRI, Dynamic State of Water, Excised Branches, Seasonal Changes, Transpiration
To cite this article
Hiromi Kano, Mika Koizumi, Seasonal Changes in the Dynamic State of Water for Excised Cherry Branches (Prunus lannesiana) Observed Using Dedicated Micro-Magnetic Resonance Imaging, Plant. Vol. 2, No. 6, 2014, pp. 60-67. doi: 10.11648/j.plant.20140206.11
F.J. Alfieri and R.F. Evert, “Seasonal development of the secondary phloem in Pinus.” American Journal of Botany, 1968. 55: 518-528.
P.B. Reich, M.B. Walters and D.S. Ellsworth, “Leaf age and season influence the relationships between leaf nitrogen, leaf mass per area and photosynthesis in maple and oak trees.” Plant, Cell and Environment, 1991. 14: 251-259.
E.J. Mellerowicz, W.K. Coleman, R.T. Riding and C.H.A. Little, “Periodicity of cambial activity in Abies balsamea. I. Effects of temperature and photoperiod on cambial dormancy and frost hardiness.” Physiologia Plantarum, 1992. 85: 515-525.
J.J. Farrar and R.F. Evert, “Seasonal changes in the ultrastructure of the vascular cambium of Robinia pseudoacacia.” Trees, 1997. 11: 191-202.
C.F. Hazlewood, D.C. Chang, D. Medina, G. Cleveland and B.L. Nichols, “Distinction between the preneoplastic and neoplastic state of murine mammary glands.” Proceedings of the National Academy of Science, USA, 1972. 69: 1478-1480.
N. Ishida, H. Kano, T. Kobayashi, H. Hamaguchi and T. Yoshida, “Estimation of biological activities by NMR in soybean seeds during maturation.” Agricultural and Biological Chemistry, 1987. 51: 301-307.
H. Kano, N. Ishida, T. Kobayashi and M. Koizumi, “1H-NMR imaging analysis of changes of free water distribution in barley and soybean seeds during maturation.” Japanese Journal of Crop Science, 1990. 59: 503-509.
P. Gaastra, “Photosynthesis of crop plants as influenced by light, carbon dioxide, temperature, and stomatal diffusion resistance.” 1959. pp. 1-68. Mededelingen, Landbouwhogeschool, 59, 13; Wageningen Veenman
M. Koizumi, N. Ishida, H. Takagishi, K. Shirata and H. Kano, “Observation of water and Na+ in tissues of the Bruguiera gymnorrhiza by 1H- and 23Na-NMR imaging.” The Botanical Magazine, Tokyo, 1992. 105: 1-11.
J.S. MacFall and G.A. Johnson, “The architecture of plant vasculature and transport as seen with magnetic resonance microscopy.” Canadian Journal of Botany, 1994. 72: 1561-1573.
N.M. Holbrook, E.T. Ahrens, M.J. Burns and M.A. Zwieniecki, “In vivo observation of cavitation and embolism repair using magnetic resonance imaging.” Plant Physiology, 2001. 126: 27-31.
M.J. Clearwater and C.J. Clark, “In vivo magnetic resonance imaging of xylem vessel contents in woody lianas.” Plant, Cell and Environment, 2003. 26, 1205–1214.
T. Umebayashi, K. Fukuda, T. Haishi, R. Sotooka, S. Zuhair and K. Otsuki, “The developmental process of xylem embolisms in pine wilt disease monitored by multipoint imaging using compact magnetic resonance imaging.” Plant Physiology, 2011. 156: 943–951.
V. De Schepper, D. van Dusschoten, P. Copini, S. Jahnke and K. Steppe, “MRI links stem water content to stem diameter variations in transpiring trees.” Journal of Experimental Botany, 2012. 63: 2645-2653.
H. Kano, N. Ishida, H. Takagishi, K. Shirata and M. Koizumi, “Tracing effects of Na+ on morphology, phosphate metabolism and accumulated compounds of a sugar beet root by NMR.” Japanese Journal of Crop Science, 1993. 62: 95-104.
C.D. Eccles, P.T. Callaghan and C.F. Jenner, “Measurement of the self-diffusion coefficient of water as a function of position in wheat grain using nuclear magnetic resonance imaging.” Biophysical Journal, 1988. 53: 77-81.
N. Ishida, H. Ogawa and H. Kano, “Diffusion of cell-associated water in ripening barley seeds.” Magnetic Resonance Imaging, 1995. 13: 745-751.
Y. Xia, V. Sarafis, E.O. Campbell and P.T. Callaghan, “Non invasive imaging of water flow in plants by NMR microscopy.” Protoplasma, 1993. 173: 170-176.
P.T. Callaghan, W. Köckenberger and J.M. Pope, “Use of difference propagators for imaging of capillary flow in the presence of stationary fluid.” Journal of Magnetic Resonance, Series B, 1994. 104: 183-188.
E. Kuchenbrod, E. Kahler, F. Thürmer, R. Deichmann, U. Zimmermann and A. Haase, “Functional magnetic resonance imaging in intact plants – quantitative observation of flow in plant vessels.” Magnetic Resonance Imaging, 1998. 16: 331-338.
M. Rokitta, U. Zimmermann and A. Haase, “Fast NMR flow measurements in plants using FLASH imaging.” Journal of Magnetic Resonance, 1999. 137: 29-32.
T.W.J. Scheenen, D. van Dusschoten, P.A. de Jager and H. Van As, “Microscopic displacement imaging with pulsed field gradient turbo spin-echo NMR.” Journal of Magnetic Resonance, 2000. 142: 207-215.
P.T. Callaghan “Principles of nuclear magnetic resonance microscopy.” 1991. Oxford Clarendon Press
W. Köckenberger, J.M. Pope, Y. Xia, K.R. Jeffrey, E. Komor and P.T. Callaghan, “A non-invasive measurement of phloem and xylem water flow in castor bean seedlings by nuclear magnetic resonance microimaging.” Planta, 1997. 201: 53-63.
C.W. Windt, F.J. Vergeldt, P.A. de Jager and H. Van As, “MRI of long-distance water transport: a comparison of the phloem and xylem flow characteristics and dynamics in poplar, castor bean, tomato and tobacco.” Plant, Cell and Environment, 2006. 29: 1715-1729.
H. Van As and J. van Duynhoven, “MRI of plants and foods.” Journal of Magnetic Resonance, 2013. 229: 25-34.
H. Kano and M. Koizumi, “Dynamic state of water in excised Ligustrum lucidum branches observed by dedicated micro-magnetic resonance imaging.” Plant, 2014. 2: 33-40. doi: 10.11648/j.plant.20140203.12.
M. Koizumi, F. Ihara, K. Yaginuma, H. Kano and T. Haishi, “Observation of the peach fruit moth, Carposina sasakii, larvae in young apple fruit by dedicated micro-magnetic resonance imaging.” Journal of Insect Science, 2010. 10: 145, available on line: insectscience.org/10.145
M. Koizumi and H. Kano, “Water entry in dry soybeans at imbibition observed by dedicated micro-magnetic resonance imaging.” American Journal of Biology and Life Sciences, 2014. 2: 6-11.
E.O. Stejskal and J.E. Tanner, “Spin diffusion measurements: spin echoes in the presence of a time-dependent field gradient.” The Journal of Chemical Physics, 1965. 42: 288-292.
N. Ishida, M. Koizumi and H. Kano, “The NMR microscope: a unique and promising tool for plant science.” 2000. Annals of Botany, 86: 259-278.
L. Murmanis, “Structural changes in the vascular cambium of Pinus strobus L. during an annual cycle.” Annals of Botany, 1971. 35: 133-141.
M. Rokitta, E. Rommel, U. Zimmermann and A. Haase, “Portable nuclear magnetic resonance imaging system.” Review of Scientific Instruments, 2000. 71: 4257–4262.
E. Danieli, J. Mauler, J. Perlo, B. Blümich and F. Casanova, “Mobile sensor for high resolution NMR spectroscopy and imaging.” Journal of Magnetic Resonance, 2009. 198: 80-87.
T. Kimura, Y. Geya, Y. Terada, K. Kose, T. Haishi, H. Gemma and Y. Sekozawa, “Development of a mobile magnetic resonance imaging system for outdoor tree measurements.” Review of Scientific Instruments, 2011, 82: 053704; doi: 10.1063/1.3589854
C.W. Windt, H. Soltner, D. van Dusschoten, and P. Blümler, “A portable Halbach magnet that can be opened and closed without force: the NMR-CUFF.” Journal of Magnetic Resonance, 2011. 208: 27-33.
T. Haishi, H. Koizumi, T. Arai, M. Koizumi and H. Kano, “Rapid detection of infestation of apple fruits by the peach fruit moth, Carposina sasakii Matsumura, larvae using a 0.2-T dedicated magnetic resonance imaging apparatus.” Applied Magnetic Resonance, 2011. 41: 1-18.
M. Jones, P.S. Aptaker, J. Cox, B.A. Gardiner and P.J. McDonald, “A transportable magnetic resonance imaging system for in situ measurements of living trees: The Tree Hugger.” Journal of Magnetic Resonance, 2012. 218: 133-140.
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