Identification of Candidate Genes and Proteins Related to the Production of ROS in Korla Pear Fruit

Aisajan Mamat; Kuerban Tusong; Juan Xu; Jixun Wang

doi:doi:10.11648/j.plant.20210901.11

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Identification of Candidate Genes and Proteins Related to the Production of ROS in Korla Pear Fruit

Aisajan Mamat, Kuerban Tusong, Juan Xu, Jixun Wang

Published in Plant (Volume 9, Issue 1)

Received: 22 December 2020 Accepted: 12 January 2021 Published: 18 January 2021

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Abstract

Korla fragrant pear (P. sinkiangensis Yü) is a landrace selected from a hybrid pear species of Xinjiang Autonomous Region in China. However, recently the formation of rough skin fruits is one of the main factors reducing fruit quality. In this study, parallel analyses of transcriptomic and proteomic data of Korla pear fruits from the three developmental stages (20, 50 and 80 days after flowering, DAF) were carried out by using RNA sequencing (RNA-seq) and tandem mass tags technology (TMT), to identify differential genes and proteins that may regulate reactive oxygen species (ROS) generation during stone cell differentiation period. In total, 42893 transcripts and 7904 proteins were acquired. Among them, 74 differentially expressed genes (DEGs) and 40 correlated proteins were identified as ROS related genes and proteins, including 15 differentially accumulated proteins (DAPs). These include genes and proteins related to the ROS production in the apoplast (24 DEGs and seven DAPs), mitochondria (23 DEGs and two DAPs), peroxisome (10 DEGs and four DAPs), and during fatty acid degradation (15 DEGs and two DAPs), respectively. All of DEGs and DAPs that related to apoplastic ROS production and some of DEGs and DAPs that related to ROS production in peroxisome and fatty acid metabolism pathways were abundantly expressed during the critical period of stone cell differentiation (20 DAF). To sum up, apoplast might be the main source of ROS production that participate in the process of stone cell differentiation in pear fruits. In addition, peroxisome and fatty acid metabolism pathways also produce certain amount of ROS during this process.

Published in	Plant (Volume 9, Issue 1)
DOI	10.11648/j.plant.20210901.11
Page(s)	1-9
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Korla Pear, Fruit Development, Transcriptome, Proteome, Reactive Oxygen Species, Stone Cells

References

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APA Style

Aisajan Mamat, Kuerban Tusong, Juan Xu, Jixun Wang. (2021). Identification of Candidate Genes and Proteins Related to the Production of ROS in Korla Pear Fruit. Plant, 9(1), 1-9. https://doi.org/10.11648/j.plant.20210901.11

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ACS Style

Aisajan Mamat; Kuerban Tusong; Juan Xu; Jixun Wang. Identification of Candidate Genes and Proteins Related to the Production of ROS in Korla Pear Fruit. Plant. 2021, 9(1), 1-9. doi: 10.11648/j.plant.20210901.11

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AMA Style

Aisajan Mamat, Kuerban Tusong, Juan Xu, Jixun Wang. Identification of Candidate Genes and Proteins Related to the Production of ROS in Korla Pear Fruit. Plant. 2021;9(1):1-9. doi: 10.11648/j.plant.20210901.11

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@article{10.11648/j.plant.20210901.11,
  author = {Aisajan Mamat and Kuerban Tusong and Juan Xu and Jixun Wang},
  title = {Identification of Candidate Genes and Proteins Related to the Production of ROS in Korla Pear Fruit},
  journal = {Plant},
  volume = {9},
  number = {1},
  pages = {1-9},
  doi = {10.11648/j.plant.20210901.11},
  url = {https://doi.org/10.11648/j.plant.20210901.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20210901.11},
  abstract = {Korla fragrant pear (P. sinkiangensis Yü) is a landrace selected from a hybrid pear species of Xinjiang Autonomous Region in China. However, recently the formation of rough skin fruits is one of the main factors reducing fruit quality. In this study, parallel analyses of transcriptomic and proteomic data of Korla pear fruits from the three developmental stages (20, 50 and 80 days after flowering, DAF) were carried out by using RNA sequencing (RNA-seq) and tandem mass tags technology (TMT), to identify differential genes and proteins that may regulate reactive oxygen species (ROS) generation during stone cell differentiation period. In total, 42893 transcripts and 7904 proteins were acquired. Among them, 74 differentially expressed genes (DEGs) and 40 correlated proteins were identified as ROS related genes and proteins, including 15 differentially accumulated proteins (DAPs). These include genes and proteins related to the ROS production in the apoplast (24 DEGs and seven DAPs), mitochondria (23 DEGs and two DAPs), peroxisome (10 DEGs and four DAPs), and during fatty acid degradation (15 DEGs and two DAPs), respectively. All of DEGs and DAPs that related to apoplastic ROS production and some of DEGs and DAPs that related to ROS production in peroxisome and fatty acid metabolism pathways were abundantly expressed during the critical period of stone cell differentiation (20 DAF). To sum up, apoplast might be the main source of ROS production that participate in the process of stone cell differentiation in pear fruits. In addition, peroxisome and fatty acid metabolism pathways also produce certain amount of ROS during this process.},
 year = {2021}
}

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TY  - JOUR
T1  - Identification of Candidate Genes and Proteins Related to the Production of ROS in Korla Pear Fruit
AU  - Aisajan Mamat
AU  - Kuerban Tusong
AU  - Juan Xu
AU  - Jixun Wang
Y1  - 2021/01/18
PY  - 2021
N1  - https://doi.org/10.11648/j.plant.20210901.11
DO  - 10.11648/j.plant.20210901.11
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 1
EP  - 9
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.20210901.11
AB  - Korla fragrant pear (P. sinkiangensis Yü) is a landrace selected from a hybrid pear species of Xinjiang Autonomous Region in China. However, recently the formation of rough skin fruits is one of the main factors reducing fruit quality. In this study, parallel analyses of transcriptomic and proteomic data of Korla pear fruits from the three developmental stages (20, 50 and 80 days after flowering, DAF) were carried out by using RNA sequencing (RNA-seq) and tandem mass tags technology (TMT), to identify differential genes and proteins that may regulate reactive oxygen species (ROS) generation during stone cell differentiation period. In total, 42893 transcripts and 7904 proteins were acquired. Among them, 74 differentially expressed genes (DEGs) and 40 correlated proteins were identified as ROS related genes and proteins, including 15 differentially accumulated proteins (DAPs). These include genes and proteins related to the ROS production in the apoplast (24 DEGs and seven DAPs), mitochondria (23 DEGs and two DAPs), peroxisome (10 DEGs and four DAPs), and during fatty acid degradation (15 DEGs and two DAPs), respectively. All of DEGs and DAPs that related to apoplastic ROS production and some of DEGs and DAPs that related to ROS production in peroxisome and fatty acid metabolism pathways were abundantly expressed during the critical period of stone cell differentiation (20 DAF). To sum up, apoplast might be the main source of ROS production that participate in the process of stone cell differentiation in pear fruits. In addition, peroxisome and fatty acid metabolism pathways also produce certain amount of ROS during this process.
VL  - 9
IS  - 1
ER  -

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Author Information

Aisajan Mamat

Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi, China
Kuerban Tusong

Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi, China
Juan Xu

Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi, China
Jixun Wang

Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi, China

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Plain Text BibTeX RIS

APA Style

Aisajan Mamat, Kuerban Tusong, Juan Xu, Jixun Wang. (2021). Identification of Candidate Genes and Proteins Related to the Production of ROS in Korla Pear Fruit. Plant, 9(1), 1-9. https://doi.org/10.11648/j.plant.20210901.11

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ACS Style

Aisajan Mamat; Kuerban Tusong; Juan Xu; Jixun Wang. Identification of Candidate Genes and Proteins Related to the Production of ROS in Korla Pear Fruit. Plant. 2021, 9(1), 1-9. doi: 10.11648/j.plant.20210901.11

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AMA Style

Aisajan Mamat, Kuerban Tusong, Juan Xu, Jixun Wang. Identification of Candidate Genes and Proteins Related to the Production of ROS in Korla Pear Fruit. Plant. 2021;9(1):1-9. doi: 10.11648/j.plant.20210901.11

Copy | Download

@article{10.11648/j.plant.20210901.11,
  author = {Aisajan Mamat and Kuerban Tusong and Juan Xu and Jixun Wang},
  title = {Identification of Candidate Genes and Proteins Related to the Production of ROS in Korla Pear Fruit},
  journal = {Plant},
  volume = {9},
  number = {1},
  pages = {1-9},
  doi = {10.11648/j.plant.20210901.11},
  url = {https://doi.org/10.11648/j.plant.20210901.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20210901.11},
  abstract = {Korla fragrant pear (P. sinkiangensis Yü) is a landrace selected from a hybrid pear species of Xinjiang Autonomous Region in China. However, recently the formation of rough skin fruits is one of the main factors reducing fruit quality. In this study, parallel analyses of transcriptomic and proteomic data of Korla pear fruits from the three developmental stages (20, 50 and 80 days after flowering, DAF) were carried out by using RNA sequencing (RNA-seq) and tandem mass tags technology (TMT), to identify differential genes and proteins that may regulate reactive oxygen species (ROS) generation during stone cell differentiation period. In total, 42893 transcripts and 7904 proteins were acquired. Among them, 74 differentially expressed genes (DEGs) and 40 correlated proteins were identified as ROS related genes and proteins, including 15 differentially accumulated proteins (DAPs). These include genes and proteins related to the ROS production in the apoplast (24 DEGs and seven DAPs), mitochondria (23 DEGs and two DAPs), peroxisome (10 DEGs and four DAPs), and during fatty acid degradation (15 DEGs and two DAPs), respectively. All of DEGs and DAPs that related to apoplastic ROS production and some of DEGs and DAPs that related to ROS production in peroxisome and fatty acid metabolism pathways were abundantly expressed during the critical period of stone cell differentiation (20 DAF). To sum up, apoplast might be the main source of ROS production that participate in the process of stone cell differentiation in pear fruits. In addition, peroxisome and fatty acid metabolism pathways also produce certain amount of ROS during this process.},
 year = {2021}
}

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TY  - JOUR
T1  - Identification of Candidate Genes and Proteins Related to the Production of ROS in Korla Pear Fruit
AU  - Aisajan Mamat
AU  - Kuerban Tusong
AU  - Juan Xu
AU  - Jixun Wang
Y1  - 2021/01/18
PY  - 2021
N1  - https://doi.org/10.11648/j.plant.20210901.11
DO  - 10.11648/j.plant.20210901.11
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 1
EP  - 9
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.20210901.11
AB  - Korla fragrant pear (P. sinkiangensis Yü) is a landrace selected from a hybrid pear species of Xinjiang Autonomous Region in China. However, recently the formation of rough skin fruits is one of the main factors reducing fruit quality. In this study, parallel analyses of transcriptomic and proteomic data of Korla pear fruits from the three developmental stages (20, 50 and 80 days after flowering, DAF) were carried out by using RNA sequencing (RNA-seq) and tandem mass tags technology (TMT), to identify differential genes and proteins that may regulate reactive oxygen species (ROS) generation during stone cell differentiation period. In total, 42893 transcripts and 7904 proteins were acquired. Among them, 74 differentially expressed genes (DEGs) and 40 correlated proteins were identified as ROS related genes and proteins, including 15 differentially accumulated proteins (DAPs). These include genes and proteins related to the ROS production in the apoplast (24 DEGs and seven DAPs), mitochondria (23 DEGs and two DAPs), peroxisome (10 DEGs and four DAPs), and during fatty acid degradation (15 DEGs and two DAPs), respectively. All of DEGs and DAPs that related to apoplastic ROS production and some of DEGs and DAPs that related to ROS production in peroxisome and fatty acid metabolism pathways were abundantly expressed during the critical period of stone cell differentiation (20 DAF). To sum up, apoplast might be the main source of ROS production that participate in the process of stone cell differentiation in pear fruits. In addition, peroxisome and fatty acid metabolism pathways also produce certain amount of ROS during this process.
VL  - 9
IS  - 1
ER  -

Copy | Download